In theory of optical aberrations, an aberrated wavefront is represented by its coefficients in some orthogonal basis, for instance by Zernike polynomials. However, many wavefront measurement techniques implicitly approximate the gradient of the …
Inhomogeneities in the refractive index of a biological microscopy sample can introduce phase aberrations, severely impairing the quality of images. Adaptive optics can be employed to correct for phase aberrations and improve image quality. However, …
With a view to the next generation of large space telescopes, we investigate guide-starfree, image-based aberration correction using a unimorph deformable mirror in a plane conjugate to the primary mirror.We designed and built a high-resolution …
The light-sheet fluorescence microscopy is an excellent tool for the investigation of large three dimensional microscopy samples at the cellular level, however, the ability to resolve features is strongly affected by the presence of scattering and …
In life sciences, interest in the microscopic imaging of increasingly complex three dimensional samples, such as cell spheroids, zebrafish embryos, and in vivo applications in small animals, is growing quickly. Due to the increasing complexity of …
We present a quantitative phase imaging microscope based on a Shack-Hartmann sensor, that directly reconstructs the optical path difference (OPD) in reflective mode. Comparing with the holographic or interferometric methods, the SH technique needs no …
In this work, we present a new confocal laser scanning microscope capable to perform sensorless wavefront optimization in real time. The device is a parallelized laser scanning microscope in which the excitation light is structured in a lattice of …
We present a new method for fast wavefront sensorless correction of phase aberrations in confocal microscopy, based on a physical model of the distribution of fluorescence light rejected by the pinhole. The method is described, and an experimental …
In this Letter, we show that a Shack-Hartmann wavefront sensor can be used for the quantitative measurement of the specimen optical path difference (OPD) in an ordinary incoherent optical microscope, if the spatial coherence of the illumination light …
Light-sheet fluorescence microscopy techniques commonly use deconvolution to remove the effect of the illumination beam shape on the image formation, reducing the effects of side lobes and increasing the contrast in three-dimensional imaging. …